Difference between revisions of "Part:BBa K1616006"

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Latest revision as of 15:42, 17 September 2015

Endolysin reversed



Endolysin works with holin; they create a toxic system, after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bläsi, 1995)

This part is the reversed sequence of the endolysin; thus this part can be used with a reversed holin, reversed promoter, reversed RBS and reversed terminator.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 169
    Illegal AgeI site found at 239
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The illegal sites have been checked


Source

This sequence is the reverses sequence of the BBa_K112806 (Endolysin).

References

Young, R., & Bläsi, U. (1995). Holins: Form and function in bacteriophage lysis. In FEMS Microbiology Reviews (Vol. 17, pp. 191–205). http://doi.org/10.1016/01686445(94)00079-4