Difference between revisions of "Part:BBa K1660004"
Line 2: Line 2:
 
<partinfo>BBa_K1660004 short</partinfo>
 
<partinfo>BBa_K1660004 short</partinfo>
 
<strong style="font-size: 150%; font-weight: bold;">  (Synthesis PCB, which can form chromophore with Cph8)</strong>
 
<strong style="font-size: 150%; font-weight: bold;">  (Synthesis PCB, which can form chromophore with Cph8)</strong>
 +
</br>
 
In order to function well, Cph8 has to form chromophore with PCB biosynthetic genes ([https://parts.igem.org/Part:BBa_I15008 BBa_I15008] and [https://parts.igem.org/Part:BBa_I15009 BBa_I15009]), where the formation of PCB requires the gene pcyA and ho1. Ho1 is a sort of heme oxygenase from <em>Synechocystis</em> which can oxidize the heme group using a ferredoxin cofactor, generating biliverdin IXalpha. And then, PcyA, a kind of ferredoxin oxidoreductase from <em>Synechocystis</em>, functions to turn biliverdin IXalpha (BV) into PCB.
 
In order to function well, Cph8 has to form chromophore with PCB biosynthetic genes ([https://parts.igem.org/Part:BBa_I15008 BBa_I15008] and [https://parts.igem.org/Part:BBa_I15009 BBa_I15009]), where the formation of PCB requires the gene pcyA and ho1. Ho1 is a sort of heme oxygenase from <em>Synechocystis</em> which can oxidize the heme group using a ferredoxin cofactor, generating biliverdin IXalpha. And then, PcyA, a kind of ferredoxin oxidoreductase from <em>Synechocystis</em>, functions to turn biliverdin IXalpha (BV) into PCB.
 
<p>We connected gene pcyA and ho1 through overlap PCR, together with the constitutive promoter ([https://parts.igem.org/Part:BBa_J23100 BBa_J23100]) to ensure a continuous work of the photoinduced system in our <em>E.coli</em>.</p>
 
<p>We connected gene pcyA and ho1 through overlap PCR, together with the constitutive promoter ([https://parts.igem.org/Part:BBa_J23100 BBa_J23100]) to ensure a continuous work of the photoinduced system in our <em>E.coli</em>.</p>

Revision as of 15:12, 17 September 2015

PcyA+Ho1 (Synthesis PCB, which can form chromophore with Cph8) </br> In order to function well, Cph8 has to form chromophore with PCB biosynthetic genes (BBa_I15008 and BBa_I15009), where the formation of PCB requires the gene pcyA and ho1. Ho1 is a sort of heme oxygenase from Synechocystis which can oxidize the heme group using a ferredoxin cofactor, generating biliverdin IXalpha. And then, PcyA, a kind of ferredoxin oxidoreductase from Synechocystis, functions to turn biliverdin IXalpha (BV) into PCB.

We connected gene pcyA and ho1 through overlap PCR, together with the constitutive promoter (BBa_J23100) to ensure a continuous work of the photoinduced system in our E.coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 816
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 369
  • 1000
    COMPATIBLE WITH RFC[1000]