Difference between revisions of "Part:BBa K1660004"

Revision as of 15:04, 17 September 2015

PcyA+Ho1 (Synthesis PCB, which can form chromophore with Cph8)

In order to function well, Cph8 has to form chromophore with PCB biosynthetic genes (<a href="https://parts.igem.org/Part:BBa_I15008">BBa_I15008</a> and <a href="https://parts.igem.org/Part:BBa_I15009">BBa_I15009</a>), where the formation of PCB requires the gene pcyA and ho1. Ho1 is a sort of heme oxygenase from Synechocystis which can oxidize the heme group using a ferredoxin cofactor, generating biliverdin IXalpha. And then, PcyA, a kind of ferredoxin oxidoreductase from Synechocystis, functions to turn biliverdin IXalpha (BV) into PCB. We connected gene pcyA（BBa_I15009）and ho1（BBa_I15008）through overlap PCR, together with the constitutive promoter(<a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>) to ensure a continuous work of the photoinduced system in our E.coli.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 816
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 369
1000
COMPATIBLE WITH RFC[1000]

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 <strong style="font-size: 150%; font-weight: bold;">  (Synthesis PCB, which can form chromophore with Cph8)</strong>
-Moreover, in order to function well, Cph8 has to form chromophore with PCB biosynthetic genes (BBa_I15008 and BBa_I15009), where the formation of PCB requires the gene pcyA and ho1. Ho1 is a sort of heme oxygenase from Synechocystis which can oxidize the heme group using a ferredoxin cofactor, generating biliverdin IXalpha. And then, PcyA, a kind of ferredoxin oxidoreductase from Synechocystis, functions to turn biliverdin IXalpha (BV) into PCB.
+In order to function well, Cph8 has to form chromophore with PCB biosynthetic genes (<a href="https://parts.igem.org/Part:BBa_I15008">BBa_I15008</a> and <a href="https://parts.igem.org/Part:BBa_I15009">BBa_I15009</a>), where the formation of PCB requires the gene pcyA and ho1. Ho1 is a sort of heme oxygenase from <em>Synechocystis</em> which can oxidize the heme group using a ferredoxin cofactor, generating biliverdin IXalpha. And then, PcyA, a kind of ferredoxin oxidoreductase from <em>Synechocystis</em>, functions to turn biliverdin IXalpha (BV) into PCB.
+We connected gene pcyA（BBa_I15009）and ho1（BBa_I15008）through overlap PCR, together with the constitutive promoter(<a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>) to ensure a continuous work of the photoinduced system in our <em>E.coli</em>.
-我们通过oe-pcr的方法，将pcyA（BBa_I15009）基因和ho1（BBa_I15008）基因进行拼接，并且加上了组成型启动子（BBa_J23100），使光控系统可以持续在大肠杆菌中发挥作用。
-We connected gene pcyA（BBa_I15009）and ho1（BBa_I15008）(through overlap PCR), together along with the constitutive promoter(BBa_J23100) to ensure a continuous work of the photoinduced system in our E.coli.(through overlap pcr这句话要放在哪儿合适啊喂？)