Difference between revisions of "Part:BBa K1696015"
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| − | + | Mf-ssrA tag can be degraded by mf-Lon, which may bring plenty of applications in all kinds of fields. However, in order to implement a more practical and quick method for detection, we incorporated tag1 (BBa_K1696013) into a RFP-based test platform in ''E.coli''. Once the protein has been expressed, it can lead to the red fluorescence detected. With the presence of mf-Lon, the red fluorescence can eventually drop off or even disappear. | |
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Latest revision as of 14:32, 17 September 2015
Mf-ssrA Tag1 reporter
Mf-ssrA tag can be degraded by mf-Lon, which may bring plenty of applications in all kinds of fields. However, in order to implement a more practical and quick method for detection, we incorporated tag1 (BBa_K1696013) into a RFP-based test platform in E.coli. Once the protein has been expressed, it can lead to the red fluorescence detected. With the presence of mf-Lon, the red fluorescence can eventually drop off or even disappear.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 555
Illegal AgeI site found at 667 - 1000COMPATIBLE WITH RFC[1000]