Difference between revisions of "Part:BBa K1696006"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1696006 short</partinfo>
 
<partinfo>BBa_K1696006 short</partinfo>
  
−
We add RFP reporter gene after P170 promoter. First, we want to test whether the inducible promoter is leaky or not. Then we use RFP gene to characterize the lactate-sensing system's performance in E.coli.
+
P170 is a acid-induced promoter, however, its trans-acting protein, RcfB, is involved in the basal activity leading to the leakage[1]. To test whether the inducible promoter is leaky or not and characterize the lactate-sensing system's performance in E.coli, we add RFP reporter gene after P170 promoter. Red fluorescent protein (rfp), isolated from a sea anemone relative (Discosoma sp.), is an ideal reporter protein for living color. Because its fluorescence can be easily detected without any pretreatment[2].
 +
 
 +
 
 +
== Reference: ==
 +
 
 +
[1] Søren M. Madsen, Thomas Hindré, Jean-Paul Le Pennec, et al. Two acid-inducible promoters from Lactococcus lactis require the cis-acting ACiD-box and the transcription regulator RcfB[J]. Molecular Microbiology, 2005, 56(3):735-746.
 +
 
 +
[2] Wan H, He J, Ju B, et al. Generation of Two-color Transgenic Zebrafish Using the Green and Red Fluorescent Protein Reporter Genes gfp and rfp[J]. Marine Biotechnology, 2002, 4(2):146-154.
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:10, 17 September 2015

P170-RFP

P170 is a acid-induced promoter, however, its trans-acting protein, RcfB, is involved in the basal activity leading to the leakage[1]. To test whether the inducible promoter is leaky or not and characterize the lactate-sensing system's performance in E.coli, we add RFP reporter gene after P170 promoter. Red fluorescent protein (rfp), isolated from a sea anemone relative (Discosoma sp.), is an ideal reporter protein for living color. Because its fluorescence can be easily detected without any pretreatment[2].


Reference:

[1] Søren M. Madsen, Thomas Hindré, Jean-Paul Le Pennec, et al. Two acid-inducible promoters from Lactococcus lactis require the cis-acting ACiD-box and the transcription regulator RcfB[J]. Molecular Microbiology, 2005, 56(3):735-746.

[2] Wan H, He J, Ju B, et al. Generation of Two-color Transgenic Zebrafish Using the Green and Red Fluorescent Protein Reporter Genes gfp and rfp[J]. Marine Biotechnology, 2002, 4(2):146-154.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 999
    Illegal AgeI site found at 1111
  • 1000
    COMPATIBLE WITH RFC[1000]