Difference between revisions of "Part:BBa K1583200"

 
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<partinfo>BBa_K1583200 short</partinfo>
 
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CsgC is positively involved in the extracellular aggregation of CsgA into amyloid nanowires (curli assembly) in E.coli functioning as a chaperone for CsgA. Constructs with different promoter strenghts were designed to investigate the impact on nanowire self-assembly.
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CsgC is positively involved in the extracellular aggregation of CsgA into amyloid nanowires (curli assembly) in E.coli. Inside the cell, it prevents the premature aggregation and thus the formation of nanowires in the cytosol. Constructs with different promoter strenghts were designed to investigate the impact of chaperone expression levels on the nanowire self-assembly process.
  
 
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Revision as of 14:00, 17 September 2015

Low constitutive promoter + CsgC

CsgC is positively involved in the extracellular aggregation of CsgA into amyloid nanowires (curli assembly) in E.coli. Inside the cell, it prevents the premature aggregation and thus the formation of nanowires in the cytosol. Constructs with different promoter strenghts were designed to investigate the impact of chaperone expression levels on the nanowire self-assembly process.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]