Difference between revisions of "Part:BBa K1824003"

(Characterization)
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===Characterization===
 
===Characterization===
 
[[File:pBad.jpg]]
 
[[File:pBad.jpg]]
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<br>'''Figure 2:''' The fluorescence of pBad+A1 with different dilution ratio at 30℃ and 37℃(Control and Arabinose).

Revision as of 13:03, 17 September 2015

pBAD + RNA Thermometer A1

This is a composite regulatory part that consisting of pBAD BBa_I13453 promoter and RNA Thermometer A1 BBa_K1824557 for temperature induced gene expression at 42 degrees. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.


A1 RNA Thermometer.jpg
Figure 1: Possible secondary structure of RNAT A1.


Characterization

PBad.jpg
Figure 2: The fluorescence of pBad+A1 with different dilution ratio at 30℃ and 37℃(Control and Arabinose).