Difference between revisions of "Part:BBa K1824000"

Revision as of 12:27, 17 September 2015

Constitutive Promoter J23119 + RNA Thermometer A1

This is a composite regulatory part that consisting of constitutive promoter BBa_J23119 and RNA Thermometer A1 BBa_K1824556 for temperature induced gene expression at 42 degrees. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.

Figure 1: Possible secondary structure of RNAT A1.

Characterization

Figure 2: The fluorescence of J23119+A1 with different dilution ratio at 30℃ and 37℃.
As Figure 2 shows, the fluorescence of the sample at 37℃ is 12 times higher than the fluorescence of the sample at 30℃, which means the amount of protein express at 37℃ is 12 times bigger than at 30℃.

References

1. Nocker, A. et al. mRNA-based thermosensor controls expression of rhizobial heat shock genes. Nucleic Acids Res. 29, 4800–4807 (2001).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 11: / Line 11: @@
 [[Image:A1-J23119.png|600px]]
 <br>'''Figure 2:''' The fluorescence of J23119+A1 with different dilution ratio at 30℃ and 37℃.
-As Figure 2 shows, the fluorescence of the sample at 37℃ is 12 times higher than the fluorescence of the sample at 30℃, which means the amount of protein express at 37℃ is 12 times bigger than at 30℃.
+<br>As Figure 2 shows, the fluorescence of the sample at 37℃ is 12 times higher than the fluorescence of the sample at 30℃, which means the amount of protein express at 37℃ is 12 times bigger than at 30℃.
 ===References===