Difference between revisions of "Part:BBa K1824000"

Revision as of 12:18, 17 September 2015

Constitutive Promoter J23119 + RNA Thermometer A1

This is a composite regulatory part that consisting of constitutive promoter BBa_J23119 and RNA Thermometer A1 BBa_K1824556 for temperature induced gene expression at 42 degrees. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.

Figure 1: Possible secondary structure of RNAT A1.

Characterization

Figure 2: The fluorescence of J23119+A1 with different dilution ratio at 30℃ and 37℃.

References

1. Nocker, A. et al. mRNA-based thermosensor controls expression of rhizobial heat shock genes. Nucleic Acids Res. 29, 4800–4807 (2001).

Sequence and Features