Difference between revisions of "Part:BBa K1769000:Experience"
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===Applications of BBa_K1769000===
 
===Applications of BBa_K1769000===
 
=='''Results'''==
 
=='''Results'''==
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<p>We test this part in BY-2 tobacoo cell line. First we construct this part in vector PSAT6. It has a promoter CAMV35S that act as a consitiutive promoter in plat cells. To see if the dimeric FYVE protein domain really binds to PI3P receptor on the endosome of plant cell, we added a Phosphoinositide-3-kinase(PI3K) inhibitor, wortmannin, that can inhibit PI3K from turning PI3P precursor into PI3P.</p>
 
<p>We test this part in BY-2 tobacoo cell line. First we construct this part in vector PSAT6. It has a promoter CAMV35S that act as a consitiutive promoter in plat cells. To see if the dimeric FYVE protein domain really binds to PI3P receptor on the endosome of plant cell, we added a Phosphoinositide-3-kinase(PI3K) inhibitor, wortmannin, that can inhibit PI3K from turning PI3P precursor into PI3P.</p>
 
<p>The picture on the left shows that after we introduce dimeric FYVE into BY-2 tobacoo cell line, the FYVE concentrated in endosome and cell membrane. However, one hour after we added 10<sup>-6</sup>M wortmannin, dimeric FYVE redistributed in cytoplasm.</p> <br>
 
<p>The picture on the left shows that after we introduce dimeric FYVE into BY-2 tobacoo cell line, the FYVE concentrated in endosome and cell membrane. However, one hour after we added 10<sup>-6</sup>M wortmannin, dimeric FYVE redistributed in cytoplasm.</p> <br>
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<figcaption>Fig123</figcaption>
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    <span class="caption">Oomycete and potato cell</span>
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<img src="https://static.igem.org/mediawiki/parts/1/1b/Nymu-fyve-wort.jpg" style="max-width:40%;"><figcaption>Fig123</figcaption></figure>
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    <span class="caption">Effector protein bonding with PI3P and entering</span>
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Revision as of 11:39, 17 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1769000

Results

<style>


div.item { 

   /* To correctly align image, regardless of content height: */
   vertical-align: top;
   display: inline-block;
   /* To horizontally center images and caption */
   text-align: center;
   /* The width of the container also implies margin around the images. */
   width: 24%;

}

.caption { 

   /* Make the caption a block so it occupies its own line. */
   display: block;
   font-weight:bold;
   font-size:16px;

} </style>




We test this part in BY-2 tobacoo cell line. First we construct this part in vector PSAT6. It has a promoter CAMV35S that act as a consitiutive promoter in plat cells. To see if the dimeric FYVE protein domain really binds to PI3P receptor on the endosome of plant cell, we added a Phosphoinositide-3-kinase(PI3K) inhibitor, wortmannin, that can inhibit PI3K from turning PI3P precursor into PI3P.

The picture on the left shows that after we introduce dimeric FYVE into BY-2 tobacoo cell line, the FYVE concentrated in endosome and cell membrane. However, one hour after we added 10-6M wortmannin, dimeric FYVE redistributed in cytoplasm.


Oomycete and potato cell
Effector protein bonding with PI3P and entering


User Reviews

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