Difference between revisions of "Part:BBa K1769000:Experience"
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===Applications of BBa_K1769000=== | ===Applications of BBa_K1769000=== | ||
| + | =='''Results'''== | ||
| + | <html> | ||
| + | <p>We test this part in BY-2 tobacoo cell line. First we construct this part in vector PSAT6. It has a promoter CAMV35S that act as a consitiutive promoter in plat cells. To see if the dimeric FYVE protein domain really binds to PI3P receptor on the endosome of plant cell, we added a Phosphoinositide-3-kinase(PI3K) inhibitor, wortmannin, that can inhibit PI3K from turning PI3P precursor into PI3P.</p> | ||
| + | <p>The picture on the left shows that after we introduce dimeric FYVE into BY-2 tobacoo cell line, the FYVE concentrated in endosome and cell membrane. However, one hour after we added 10<sup>-6</sup>M wortmannin, dimeric FYVE redistributed in cytoplasm.</p> <br> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/7/75/Nymu-fyve.jpg" style="padding-left:15%;max-width:40%;"> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/1/1b/Nymu-fyve-wort.jpg" style="max-width:40%;"></html> | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 10:45, 17 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1769000
Results
We test this part in BY-2 tobacoo cell line. First we construct this part in vector PSAT6. It has a promoter CAMV35S that act as a consitiutive promoter in plat cells. To see if the dimeric FYVE protein domain really binds to PI3P receptor on the endosome of plant cell, we added a Phosphoinositide-3-kinase(PI3K) inhibitor, wortmannin, that can inhibit PI3K from turning PI3P precursor into PI3P.
The picture on the left shows that after we introduce dimeric FYVE into BY-2 tobacoo cell line, the FYVE concentrated in endosome and cell membrane. However, one hour after we added 10-6M wortmannin, dimeric FYVE redistributed in cytoplasm.
User Reviews
UNIQfe66d3e67d6e4626-partinfo-00000001-QINU UNIQfe66d3e67d6e4626-partinfo-00000002-QINU