Difference between revisions of "Part:BBa K1686033:Experience"
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===Applications of BBa_K1686033=== | ===Applications of BBa_K1686033=== | ||
To verify <i>OsmY</i> promoter is only active in the stationary phase, we realized Curdlan quantitative analysis every hour of a culture in LB medium. The switch of temperature for the culture is linked to the transition in stationary phase. | To verify <i>OsmY</i> promoter is only active in the stationary phase, we realized Curdlan quantitative analysis every hour of a culture in LB medium. The switch of temperature for the culture is linked to the transition in stationary phase. | ||
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| − | + | <br>→ As we can see, Curdlan appears after the switch at 27°C. So, <i>OsmY</i> promoter is active in stationary phase only. <b>(Fig.7)<b></p> | |
| − | + | https://static.igem.org/mediawiki/2015/thumb/1/16/Bordeaux_Team_promoter_characterizationV4.png/800px-Bordeaux_Team_promoter_characterizationV4.png | |
| − | + | <p class="reference" align ="center"> <b> Figure 7: <i>OsmY</i> promoter characterization <br> </p> | |
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===User Reviews=== | ===User Reviews=== | ||
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Revision as of 09:31, 17 September 2015
Applications of BBa_K1686033
To verify OsmY promoter is only active in the stationary phase, we realized Curdlan quantitative analysis every hour of a culture in LB medium. The switch of temperature for the culture is linked to the transition in stationary phase.
→ As we can see, Curdlan appears after the switch at 27°C. So, OsmY promoter is active in stationary phase only. (Fig.7)<b></p>
<b> Figure 7: OsmY promoter characterization
User Reviews
UNIQd401f1bbe0bc56ef-partinfo-00000000-QINU UNIQd401f1bbe0bc56ef-partinfo-00000001-QINU