Difference between revisions of "Part:BBa K1686033:Experience"
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===Applications of BBa_K1686033=== | ===Applications of BBa_K1686033=== | ||
| − | + | To verify <i>OsmY</i> promoter is only active in the stationary phase, we realized Curdlan quantitative analysis every hour of a culture in LB medium. The switch of temperature for the culture is linked to the transition in stationary phase. | |
| + | <br>→ As we can see, Curdlan appears after the switch at 27°C. So, <i>OsmY</i> promoter is active in stationary phase only. <b>(Fig.7)<b></p> | ||
| + | <img style="position:relative;left:25%;width:45vw;height:30vw" src="https://static.igem.org/mediawiki/2015/thumb/1/16/Bordeaux_Team_promoter_characterizationV4.png/800px-Bordeaux_Team_promoter_characterizationV4.png"> | ||
| + | <p class="reference" align ="center"> <b> Figure 7: <i>OsmY</i> promoter characterization <br> </p> | ||
| + | <br> | ||
===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_K1686033 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K1686033 StartReviews</partinfo> | ||
Revision as of 09:31, 17 September 2015
Applications of BBa_K1686033
To verify OsmY promoter is only active in the stationary phase, we realized Curdlan quantitative analysis every hour of a culture in LB medium. The switch of temperature for the culture is linked to the transition in stationary phase.
→ As we can see, Curdlan appears after the switch at 27°C. So, OsmY promoter is active in stationary phase only. (Fig.7)<b></p> <img style="position:relative;left:25%;width:45vw;height:30vw" src="">
<b> Figure 7: OsmY promoter characterization
User Reviews
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