Difference between revisions of "Part:BBa K1592022:Design"
WangjieLiu (Talk | contribs) (→Source) |
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===Source=== | ===Source=== | ||
| − | + | Cloned from BBa_K588001 and mutated by overlap extension PCR. | |
===References=== | ===References=== | ||
Latest revision as of 05:42, 17 September 2015
ptrp mutant3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
As the trp promoter has two illegal restriction enzyme sites (SpeI),we designed the point mutantion to improve it.
Source
Cloned from BBa_K588001 and mutated by overlap extension PCR.