Difference between revisions of "Part:BBa K1632001:Design"

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__NOTOC__
 
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<partinfo>BBa_K1632001 short</partinfo>
 
<partinfo>BBa_K1632001 short</partinfo>
  
 
<partinfo>BBa_K1632001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1632001 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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sequence confirmed
 
sequence confirmed
  
 
===Materials and Methods===
 
===Materials and Methods===
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 +
<strong>Please click  [https://parts.igem.org/Part:BBa_K1632003:Experience here]</strong>. (fim switch[default OFF](Tokyo_Tech/J23119)_rbs_GFP Experiment Page)
  
 
===Source===
 
===Source===

Revision as of 05:09, 17 September 2015

fim switch[default OFF](Tokyo_Tech/J23119)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 98
    Illegal NheI site found at 121
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 92
    Illegal BamHI site found at 133
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

sequence confirmed

Materials and Methods

Please click here. (fim switch[default OFF](Tokyo_Tech/J23119)_rbs_GFP Experiment Page)

Source

PCR after the invertion experiment of BBa_K1632000

References

Ian C. Blomfield et al. (1997) Integration host factor stimulates both FimB- andFimE-mediated site-specific DNA inversion that controlsphase variation of type 1 fimbriae expression in Escherichia coli. Molecular Microbiology 23(4), 705–717

John M. Abraham et al. (1985) An invertible element of DNA controls phase variation of type 1 fimbriae of Escherichia coli. Proc Natl Acad Sci U S A 82(17):5724-7

Matthew P. McCusker et al. (2008) DNA sequence heterogeneity in Fim tyrosine-integrase recombinase-binding elements and functional motif asymmetries determine the directionality of the fim genetic switch in Escherichia coli K-12. Molecular Microbiology 67(1): 171–187