Difference between revisions of "Part:BBa K1592009:Design"
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===Source=== | ===Source=== | ||
| − | E. coli ribosomal protein L2 was synthesized by IDT, LIP2 prepro and YLcwp3 were cloning from the plasmid JMP62, and we fused this three sequences. | + | E. coli ribosomal protein L2 was synthesized by IDT, LIP2 prepro and GS-linker-YLcwp3 were cloning from the plasmid JMP62, and we fused this three sequences. |
===References=== | ===References=== | ||
Latest revision as of 04:50, 17 September 2015
LIP2 prepro + E. coli ribosomal protein L2 (203-273aa) + YLcwp3 Fusion
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 100
Illegal XhoI site found at 410 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 431
Design Notes
In order to replace the domains more conveniently, we respectivey added BamHI, SalI, NdeI between LIP2 prepro, E. coli ribosomal protein L2 (203-273aa), GS linker and YLcwp3.Also we added 6Xhis-tag between LIP2 prepro and E. coli ribosomal protein L2 (203-273aa)to do verification of immunofluorescence.
Source
E. coli ribosomal protein L2 was synthesized by IDT, LIP2 prepro and GS-linker-YLcwp3 were cloning from the plasmid JMP62, and we fused this three sequences.