Difference between revisions of "Part:BBa K1592008:Design"

(→‎Design Notes)
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1592008 short</partinfo>
 
<partinfo>BBa_K1592008 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
In order to replace the domains more conveniently, we added BamHI, SalI, NdeI, NdeI between LIP2 prepro, E. coli ribosomal protein L2, GS linker and YLcwp3.Also we added 6Xhis-tag between  LIP2 prepro and E. coli ribosomal protein L2 to do verification of immunofluorescence.
+
In order to replace the domains more conveniently, we added BamHI, SalI, NdeI, NdeI between LIP2 prepro, E. coli ribosomal protein L2(61-222aa), GS linker and YLcwp3.Also we added 6Xhis-tag between  LIP2 prepro and E. coli ribosomal protein L2 (61-222aa) to do verification of immunofluorescence.
  
 
===Source===
 
===Source===

Latest revision as of 04:46, 17 September 2015

LIP prepro + E. coli ribosomal protein L2 (61-202aa) + YLcwp3 Fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 100
    Illegal XhoI site found at 620
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 641


Design Notes

In order to replace the domains more conveniently, we added BamHI, SalI, NdeI, NdeI between LIP2 prepro, E. coli ribosomal protein L2(61-222aa), GS linker and YLcwp3.Also we added 6Xhis-tag between LIP2 prepro and E. coli ribosomal protein L2 (61-222aa) to do verification of immunofluorescence.

Source

E. coli ribosomal protein L2 was synthesized by IDT, LIP2 prepro and YLcwp3 were cloning from the plasmid JMP62, and we fused this three sequences.

References