Difference between revisions of "Part:BBa K1592007:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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In order to replace more convenient, we respectively added BamHI, SalI, NdeI between  LIP2 prepro, E. coli ribosomal protein L2 (1-60), GS linker, YLcwp3.Also we add the 6Xhis-tag between  LIP2 prepro and E. coli ribosomal protein L2 (1-60) to do verification of immunofluorescence.
+
In order to replace more convenient, we respectively added BamHI, SalI, NdeI between  LIP2 prepro, E. coli ribosomal protein L2 (1-60), GS linker, YLcwp3.Also we added the 6Xhis-tag between  LIP2 prepro and E. coli ribosomal protein L2 (1-60) to do verification of immunofluorescence.
  
 
===Source===
 
===Source===

Latest revision as of 04:43, 17 September 2015


LIP prepro + E. coli ribosomal protein L2 (1-60aa) + YLcwp3 Fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 100
    Illegal XhoI site found at 374
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 395


Design Notes

In order to replace more convenient, we respectively added BamHI, SalI, NdeI between LIP2 prepro, E. coli ribosomal protein L2 (1-60), GS linker, YLcwp3.Also we added the 6Xhis-tag between LIP2 prepro and E. coli ribosomal protein L2 (1-60) to do verification of immunofluorescence.

Source

E. coli ribosomal protein L2 was synthesized by IDT. LIP2 prepro and YLcwp3 were cloning from the plasmid JMP62 of our lab.

References