Difference between revisions of "Part:BBa K1732010"

Revision as of 04:35, 17 September 2015

pSB1C3-J23100-B0034-E0040-His-B0015

His tagged E0040 GFP and strong RBS. The J23100 strong constitutive promoter allows for high level expression of the fluorescent protein used to measure fluorescence.

A set of fluorescent proteins which includes BFP, GFP, eGFP, E0040 GFP, OFP, YFP, and RFP with a J23100 constitutive promoter and RFP with a J23115 constitutive promoter were characterized. The relative fluorescence as well as the signal-to-noise ratio of all the proteins in the MACH competent cell line were calculated. eGFP was measured at (ex/em = 501nm/511nm).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 212
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 77

@@ Line 9: / Line 9: @@
 A set of fluorescent proteins which includes BFP, GFP, eGFP, E0040 GFP, OFP, YFP, and RFP with a J23100 constitutive promoter and RFP with a J23115 constitutive promoter were characterized. The relative fluorescence as well as the signal-to-noise ratio of all the proteins in the MACH competent cell line were calculated. eGFP was measured at (ex/em = 501nm/511nm).
+[[File:FIntensity.jpg]]
+[[File:FQuantitation.jpg]]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===