Difference between revisions of "Part:BBa K1820014"

Revision as of 04:25, 17 September 2015

CP8_RBS_sfGFP(Bs)_Terminator

Usage and Biology

This is a composite part designed as a report protein sequence for use in Lactococcus lactis. The promoter is a mid-range promoter from Peter Ruhdal Jensen and Karin Hammer's library of synthetic promoters for Lactococcus lactis followed by a popular ribosome binding site (Elowitz 1999), a super folded green fluorescent protein codon optimized for Bacillus subtilis (sfGFP(Bs)), and a popular double-stop terminator. Although it was designed for L. lactis, it has displayed function in Escherichia coli. The sfGFP(Bs) has worked well in a variety of bacteria, such as L. lactis, B. subtilis, Escherischia coli and Streptococcus pneumoniae and there are indications that the promoter is functional in a large number of prokaryotic organisms as well, so it is likely that this construct will be functional in a variety of prokaryotic organisms.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 99

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 ===Usage and Biology===
-<p> This is a composite part designed as a report protein sequence for use in ''Lactococcus lactis''. The promoter is a mid-range promoter from Peter Ruhdal Jensen and Karin Hammer's library of synthetic promoters for ''Lactococcus lactis'' followed by a popular ribosome binding site (Elowitz 1999), a super folded green fluorescent protein codon optimized for ''Bacillus subtilis'' (sfGFP(Bs)), and a popular double-stop terminator. Although it was designed for ''L. lactis'', it has displayed function in ''Escherichia coli''. The sfGFP(Bs) has worked well in a variety of bacteria, such as ''L. lactis', ''B. subtilis'', ''Escherischia coli'' and ''Streptococcus pneumoniae'' and there are indications that the promoter is functional in a large number of prokaryotic organisms as well, so it is likely that this construct will be functional in a variety of prokaryotic organisms.</p>
+<p> This is a composite part designed as a report protein sequence for use in ''Lactococcus lactis''. The promoter is a mid-range promoter from Peter Ruhdal Jensen and Karin Hammer's library of synthetic promoters for ''Lactococcus lactis'' followed by a popular ribosome binding site (Elowitz 1999), a super folded green fluorescent protein codon optimized for ''Bacillus subtilis'' (sfGFP(Bs)), and a popular double-stop terminator. Although it was designed for ''L. lactis'', it has displayed function in ''Escherichia coli''. The sfGFP(Bs) has worked well in a variety of bacteria, such as ''L. lactis'', ''B. subtilis'', ''Escherischia coli'' and ''Streptococcus pneumoniae'' and there are indications that the promoter is functional in a large number of prokaryotic organisms as well, so it is likely that this construct will be functional in a variety of prokaryotic organisms.</p>
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 <span class='h3bb'>Sequence and Features</span>