Difference between revisions of "Part:BBa K1732007"

Revision as of 04:14, 17 September 2015

pSB1C3-J23100-B0034-BFP-His-B0015

His tagged mTagBFP and strong RBS. The J23100 strong constitutive promoter allows for high level expression of the fluorescent protein used to measure fluorescence.

A set of fluorescent proteins which includes BFP, GFP, eGFP, E0040, OFP, YFP, and RFP with a J23100 constitutive promoter and RFP with a J23115 constitutive promoter were characterized. The relative fluorescence as well as the signal-to-noise ratio of all the proteins in the MACH competent cell line were calculated. BFP was measured at (ex/em = 399nm/456).

File:Intensity.jpg

File:Quantitation.jpg

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 285
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 607
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 518
Illegal BsaI.rc site found at 683

@@ Line 9: / Line 9: @@
 A set of fluorescent proteins which includes BFP, GFP, eGFP, E0040, OFP, YFP, and RFP with a J23100 constitutive promoter and RFP with a J23115 constitutive promoter were characterized. The relative fluorescence as well as the signal-to-noise ratio of all the proteins in the MACH competent cell line were calculated. BFP was measured at (ex/em = 399nm/456).
+[[File:Intensity.jpg]]
+[[File:Quantitation.jpg]]
 <!-- Add more about the biology of this part here