Difference between revisions of "Part:BBa K1819004"

Revision as of 01:59, 17 September 2015

GFP+TetR+pTetR+mRFP1

This construction gives the possibility of adding the most suitable promoter in order to regulate GFP transcription and any genetic sequence inserted after RBS and before TetR. In the absence of tetracycline bound in the tetR site, the TetR promoter (BBa_R0040) transcripts the RFP (or the gene located in the region corresponding to RFP).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2211
Illegal AgeI site found at 2323
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 644

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1819004 short</partinfo>
-This construction gives the possibility of adding the most suitable promoter in order to regulate GFP transcription and any genetic sequence inserted after RBS and before TetR. In the absence of tetracycline bound in the tetR site, the promoter tetR (part R0040) transcripts the RFP (or the gene located in the region corresponding to RFP).
+This construction gives the possibility of adding the most suitable promoter in order to regulate GFP transcription and any genetic sequence inserted after RBS and before TetR. In the absence of tetracycline bound in the tetR site, the TetR promoter  (BBa_R0040) transcripts the RFP (or the gene located in the region corresponding to RFP).
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