Difference between revisions of "Part:BBa J23117"

Revision as of 23:06, 16 September 2015

constitutive promoter family member

 Variant RFP (au)
 J23112           1
 J23103           17
 J23113           21
 J23109           106
 J23117           162
 J23114           256
 J23115           387
 J23116           396
 J23105           623
 J23110           844
 J23107           908
 J23106           1185
 J23108           1303
 J23118           1429
 J23111           1487
 J23101           1791
 J23104           1831
 J23102           2179
 J23100           2547

Constitutive promoter family
Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. J23119 is the "consensus" promoter sequence and the strongest member of the family. All parts except J23119 are present in plasmid J61002. Part J23119 is present in pSB1A2. This places the RFP downstream of the promoter. Reported activities of the promoters are given as the relative fluorescence of these plasmids in strain TG1 grown in LB media to saturation. See part BBa_J61002 for details on their use.

These promoter parts can be used to tune the expression level of constitutively expressed parts. The NheI and AvrII restriction sites present within these promoter parts make them a scaffold for further modification. JCAraw

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

This promoter was BioBricked in 2015 competition with a PAM rich Upstream regulation sequence (URS), BBa_K1723001. This PAM rich sequence is needed for the protein dCas9 to bind to the promoter and act as a programmable transcription regulator . Another promoter, PAM rich URS J23117Alt ( BBa_K1723005 ) was created by mutating sequences of the improved J23117 promoter keeping intact -10 sequence and -35 sequence to keep a similar promoter strength.

@@ Line 11: / Line 11: @@
 <partinfo>BBa_J23117 SequenceAndFeatures</partinfo>
-This promoter was BioBricked in 2015 competition with a PAM rich Upstream regulation sequence (URS), BBa_K1723001. This PAM rich sequence is needed for the protein dCas9 to bind to the promoter to act as a programmable transcription regulator with specific sgRNAs. Another promoter, PAM rich URS J23117Alt ( BBa_K1723005 ) was created by mutated sequences of the improved J23117 promoter keeping intact -10 sequence and -35 sequence to keep a similar promoter strength.
+This promoter was BioBricked in 2015 competition with a PAM rich Upstream regulation sequence (URS), BBa_K1723001. This PAM rich sequence is needed for the protein dCas9 to bind to the promoter and act as a programmable transcription regulator . Another promoter, PAM rich URS J23117Alt ( BBa_K1723005 ) was created by mutating sequences of the improved J23117 promoter keeping intact -10 sequence and -35 sequence to keep a similar promoter strength.
 <!-- Uncomment this to enable Functional Parameter display