Difference between revisions of "Part:BBa K1819004"

 
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<partinfo>BBa_K1819004 short</partinfo>
 
<partinfo>BBa_K1819004 short</partinfo>

Revision as of 21:06, 16 September 2015

GFP+TetR+pTetR+mRFP1

This construction gives the possibility of adding the most suitable promoter in order to regulate GFP transcription and any genetic sequence inserted after RBS and before TetR. In the absence of tetracycline bound in the tetR site, the promoter tetR (part R0040) transcripts the RFP (or the gene located in the region corresponding to RFP).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2211
    Illegal AgeI site found at 2323
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644