Difference between revisions of "Part:BBa K1725040"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1725040 short</partinfo>
 
<partinfo>BBa_K1725040 short</partinfo>
−
 
−
PhlF repressor from Pseudomonas protegens Pf-5. Represses promoter K1725000.
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 12: Line 9:
 
<partinfo>BBa_K1725040 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1725040 SequenceAndFeatures</partinfo>
  
 +
K1725000 (PhlF repressible promoter) driven expression is repressed by K1725040 as shown in the figure below. K1725042 is K1725040 driven by the <i>lacI</i> regulated promoter K1725080. Our control was K1725083 (the Tet repressor C0040 also driven by K1725080) and K1725082 (the TetR repressible promoter R0040 driving expression of I13500).
 +
 +
 +
https://static.igem.org/mediawiki/2015/9/95/Glasgow_2015_Repression_Fold_Graph.png
 +
 +
<b>Represser constructs with pSB1C3 backbone; promoter driving GFP constructs with pSB3K3 backbone. Repressor protein expression induced with 100μM IPTG. Replicates of constructs and controls of three dilutions from one colony, under the same conditions. Mean and standard deviation of replicates were calculated to give value and error bars.</b>
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 20:30, 16 September 2015

phlF encoding PhlF repressor from Pseudomonas protegens Pf-5

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

K1725000 (PhlF repressible promoter) driven expression is repressed by K1725040 as shown in the figure below. K1725042 is K1725040 driven by the lacI regulated promoter K1725080. Our control was K1725083 (the Tet repressor C0040 also driven by K1725080) and K1725082 (the TetR repressible promoter R0040 driving expression of I13500).


Glasgow_2015_Repression_Fold_Graph.png

Represser constructs with pSB1C3 backbone; promoter driving GFP constructs with pSB3K3 backbone. Repressor protein expression induced with 100μM IPTG. Replicates of constructs and controls of three dilutions from one colony, under the same conditions. Mean and standard deviation of replicates were calculated to give value and error bars.