Difference between revisions of "Part:BBa K1679012:Design"
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===References=== | ===References=== | ||
Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches[J]. Nature Reviews Microbiology, 2012, 10(4):255-265. | Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches[J]. Nature Reviews Microbiology, 2012, 10(4):255-265. | ||
Revision as of 19:53, 16 September 2015
promoter+RNA thermometer+RFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 713
Illegal AgeI site found at 825 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use a special RBS to make the device temperature-sensitive.When the temperature is below 37°C,the secondary structure of the RBS after transform would be folded.Thus the ribosome can't translated RFP.And when the temperature rises above 37°C,the secondary structure of the RBS after transform would be unfolded and produce RFP.However,according to our experience,it didn't appear red when the temperature rises above 37°C.
Source
BBa_J23106
BBa_K115003
BBa_E1010
BBa_B0015
References
Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches[J]. Nature Reviews Microbiology, 2012, 10(4):255-265.