Difference between revisions of "Part:BBa K1592025"

Latest revision as of 15:09, 16 September 2015

ptrp mutant3+RBS+GFP

Ptrp(BBa_K191007) is a promoter with the trp operator. It will be repressed by trpR (BBa_K588000) and LovTAP.

Note that the original trp operator contains two SpeI sites.

We remove the illegal sites without affecting its function by site-directed mutagenesis for meeting the requirements of RFC10 and more convenient to be assembled, and finally constructed three ptrp muant, called ptrp mutant1(BBa_K1592020), ptrp mutant2(BBa_K1592021), and ptrp mutant3(BBa_K1592022).

This composite is used to validating if the mutants would affect the function.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 756

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1592025 short</partinfo>
-ptrp is a promoter with the trp operator. It will be repressed by trpR (K588000) and LovTAP.
+Ptrp([https://parts.igem.org/Part:BBa_K191007 BBa_K191007]) is a promoter with the trp operator. It will be repressed by trpR ([https://parts.igem.org/Part:BBa_K588000 BBa_K588000]) and LovTAP.
-Note that the original trp operator contains two SpeI sites&#12290;
+<p>Note that the original trp operator contains two SpeI sites.</p>
-We remove the illegal sites without affecting its function by site-directed mutagenesis for meeting the requirements of RFC10 and more convenient to be assembled, and finally constructed three ptrp muant, called ptrp mutant1, ptrp mutant2, and ptrp mutant3.
+<p>We remove the illegal sites without affecting its function by site-directed mutagenesis for meeting the requirements of RFC10 and more convenient to be assembled, and finally constructed three ptrp muant, called ptrp mutant1([https://parts.igem.org/Part:BBa_K1592020 BBa_K1592020]), ptrp mutant2([https://parts.igem.org/Part:BBa_K1592021 BBa_K1592021]), and ptrp mutant3([https://parts.igem.org/Part:BBa_K1592022 BBa_K1592022]).
-In order to test the mutants, we construct the RBS and GFP after them.
+</p>
+[[File: ptrp_mutants.png|700px|thumb|center|]]
+This composite is used to validating if the mutants would affect the function.
 <!-- Add more about the biology of this part here