Difference between revisions of "Part:BBa M31502:Design"

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==Changes: Where and Why==
 
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#bp 415-420: want to remove Gene 10 (-10) promoter binding sequence
 
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#*frame = TTG ACA : Leu Thr
 
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#**change to = CTG  ACC : Leu Thr
 
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#Cut gene 2 at HpaI site. HpaI site will be a boundary for section A of M13.1 refactored genome
 
  
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==New 0-cutter site==
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__NOTOC__
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#AatII at end
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<partinfo>BBa_M31502 short</partinfo>
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<partinfo>BBa_M31502 SequenceAndFeatures</partinfo>
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===Design Notes===
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Want to eliminate 2ndary functions
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===Source===
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BBa_M13002 from M13 phage
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===References===

Revision as of 05:27, 24 February 2007


BBa_M13002 modified to remove g10 promoter function


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Want to eliminate 2ndary functions


Source

BBa_M13002 from M13 phage

References