Difference between revisions of "Part:BBa K1592020"

Revision as of 14:45, 16 September 2015

Ptrp mutant1

Ptrp(BBa_K191007 BBa_K191007) is a promoter with the trp operator. It will be repressed by trpR (https://parts.igem.org/Part: BBa_K588000 BBa_K588000) and LovTAP. Note that the original trp operator contains two SpeI sites。 We remove the illegal sites without affecting its function by site-directed mutagenesis for meeting the requirements of RFC10 and more convenient to be assembled, and finally constructed three ptrp muant, called ptrp mutant1, ptrp mutant2, and ptrp mutant3.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 14:44, 16 September 2015 (view source) ShuyanTang (Talk \| contribs) ← Older edit		Revision as of 14:45, 16 September 2015 (view source) ShuyanTang (Talk \| contribs) Newer edit →
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	We remove the illegal sites without affecting its function by site-directed mutagenesis for meeting the requirements of RFC10 and more convenient to be assembled, and finally constructed three ptrp muant, called ptrp mutant1, ptrp mutant2, and ptrp mutant3.		We remove the illegal sites without affecting its function by site-directed mutagenesis for meeting the requirements of RFC10 and more convenient to be assembled, and finally constructed three ptrp muant, called ptrp mutant1, ptrp mutant2, and ptrp mutant3.

−	[[File: ptrp_mutants.png\|~~400px~~\|thumb\|center\|]]	+	[[File: ptrp_mutants.png\|700px\|thumb\|center\|]]