Difference between revisions of "Part:BBa K1592020"
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<partinfo>BBa_K1592020 short</partinfo> | <partinfo>BBa_K1592020 short</partinfo> | ||
| − | + | Ptrp([https://parts.igem.org/Part: BBa_K191007 BBa_K191007]) is a promoter with the trp operator. It will be repressed by trpR (https://parts.igem.org/Part: BBa_K588000 BBa_K588000) and LovTAP. | |
| − | Note that the original trp operator contains two SpeI | + | Note that the original trp operator contains two SpeI sites。 |
We remove the illegal sites without affecting its function by site-directed mutagenesis for meeting the requirements of RFC10 and more convenient to be assembled, and finally constructed three ptrp muant, called ptrp mutant1, ptrp mutant2, and ptrp mutant3. | We remove the illegal sites without affecting its function by site-directed mutagenesis for meeting the requirements of RFC10 and more convenient to be assembled, and finally constructed three ptrp muant, called ptrp mutant1, ptrp mutant2, and ptrp mutant3. | ||
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| + | [[File: ptrp_mutants.png|400px|thumb|center|]] | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 14:44, 16 September 2015
Ptrp mutant1
Ptrp(BBa_K191007 BBa_K191007) is a promoter with the trp operator. It will be repressed by trpR (https://parts.igem.org/Part: BBa_K588000 BBa_K588000) and LovTAP. Note that the original trp operator contains two SpeI sites。 We remove the illegal sites without affecting its function by site-directed mutagenesis for meeting the requirements of RFC10 and more convenient to be assembled, and finally constructed three ptrp muant, called ptrp mutant1, ptrp mutant2, and ptrp mutant3.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]