Difference between revisions of "Part:BBa K1655001"

Revision as of 14:30, 16 September 2015

This GFP can be fused into any protein's aminoterminal end with the BioBrick enzyme assembly method.

An extra nucelotide has been added prior to this GFP brick's suffix to maintain the reading frame after fusion.

Usage and Biology

This part sequence is derived from https://parts.igem.org/Part:BBa_I13504. The part has been PCRed from BBa_I13504, and the reverse primer has added an extra guanine nucleotide prior to the birck's suffix. This will allow for easy aminoterminal GFP fusion with the biobrick enzyme assembly.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 644

@@ Line 4: / Line 4: @@
 An extra nucelotide has been added prior to this GFP brick's suffix to maintain the reading frame after fusion.
-<!-- Add more about the biology of this part here
+<!-- Add more about the biology of this part here -->
 ===Usage and Biology===
 This part sequence is derived from https://parts.igem.org/Part:BBa_I13504. The part has been PCRed from BBa_I13504, and the reverse primer has added an extra guanine nucleotide prior to the birck's suffix. This will allow for easy aminoterminal GFP fusion with the biobrick enzyme assembly.
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 <span class='h3bb'>Sequence and Features</span>