Difference between revisions of "Part:BBa K1655000"

Revision as of 12:10, 16 September 2015

Propane 1 codes three of the ten enzymes necessary to produce propane in Escherichia coli.

Propane 1 includes 1)acyl-CoA thioester hydrolase (YciA) from Haemophilus influenzae, which catalyzes the reaction: acyl-CoA + H2O <-> CoA + a carboxylate, 2) Carboxylic acid reductase (CAR) from Mycobacterium marinum (strain ATCC BAA-535 / M), which catalyzes the reaction: a carboxylate + reduced acceptor <-> an aldehyde + acceptor + H2O, and 3) maturation factor phosphopantetheinyl transferase (sfp) from Bacillus subtilis, which activates CAR by catalyzing the transfer of a prosthetic phosphopanteine group to CAR.

All coding regions are assembled as an operon after a T7 promoter and and RBS from common cloning vectors (such as pCDF-Duet1 by Novagen). Terminator sequence after the last coding sequence, CAR is also derived from common cloning vectors (e.g. pCDF-Duet1 by Novagen uses this terminator).

All coding sequences have been codon optimized twice for E. coli. First with Thermo Fisher's codon optimizer and then with IDT's codon optimization software.

Propane 1 can be used as a part to produce propane in E. coli. The propane production pathway is shown below, and the enzymes included in Propane 1 are highlighted in blue.

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Sequence and Features