Difference between revisions of "Part:BBa M31022:Design"
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__NOTOC__
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<partinfo>BBa_M31022 short</partinfo>
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<partinfo>BBa_M31022 SequenceAndFeatures</partinfo>
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===Design Notes===
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<tt>
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I wanted to weaken RBS X and preserve II AA sequence.
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II:              gca ttt gag ggg gat tca
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X RBS:            gca ttt gag ggg gat tca
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AA seq:          ALA PHE GLU GLY ASP SER
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                        -10^
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X.1 RBS region:  gcg ttc gaa ggc gac tcg
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New AA seq:      ALA PHE GLU GLY ASP SER
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===Source===
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gene II
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===References===

Revision as of 08:48, 28 February 2007


Gene II (RBS X.1 silent mutant)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1234
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1234
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1234
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1234
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

I wanted to weaken RBS X and preserve II AA sequence.

II: gca ttt gag ggg gat tca X RBS: gca ttt gag ggg gat tca AA seq: ALA PHE GLU GLY ASP SER 

                       -10^

X.1 RBS region: gcg ttc gaa ggc gac tcg New AA seq: ALA PHE GLU GLY ASP SER



Source

gene II

===References===