Difference between revisions of "Part:BBa I13001"

Revision as of 09:52, 16 September 2015

RBS B0030 w/ YFP (-LVA) TT

B0030. E0030. B0015

Glasgow iGEM 2011

This part was found to express well when excited with fluorescence in the range of 515-565nm. See images below for expression testing:

Shenzhen_SFLS (IGEM 2015)

We expressed this part in E.coli using pbad promoter and J23116 promoter.

From left to right: J23116/pbad(0μM L-Arabinose)/1.0μM/2.0μM/4.0μM(best in the 5 concentrations we usd)/8.0μM File:QQ图片20150210175650.jpeg.jpg File:QQ图片20150210175655.jpeg.jpg

Sequence and Features

Assembly Compatibility:

Revision as of 09:51, 16 September 2015 (view source) VictorX (Talk \| contribs) (→‎Experience) ← Older edit		Revision as of 09:52, 16 September 2015 (view source) VictorX (Talk \| contribs) (→‎Experience) Newer edit →
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	From left to right: J23116/pbad(0μM L-Arabinose)/1.0μM/2.0μM/4.0μM(best in the 5 concentrations we usd)/8.0μM		From left to right: J23116/pbad(0μM L-Arabinose)/1.0μM/2.0μM/4.0μM(best in the 5 concentrations we usd)/8.0μM
−	[[File:QQ图片20150210175650.jpeg]] [[File:QQ图片20150210175655.jpeg]]	+	[[File:QQ图片20150210175650.jpeg.jpg]] [[File:QQ图片20150210175655.jpeg.jpg]]