Difference between revisions of "Part:BBa I13001"
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[[Image:Gla_YFP_Results.jpg]] | [[Image:Gla_YFP_Results.jpg]] | ||
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+ | Shenzhen_SFLS (IGEM 2015) | ||
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+ | We expressed this part in E.coli using pbad promoter and J23116 promoter. | ||
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+ | From left to right: J23116/pbad(0μM L-Arabinose)/1.0μM/2.0μM/4.0μM(best in the 5 concentrations we usd)/8.0μM | ||
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+ | [[Image:QQ图片20150210175650.jpeg]] | ||
+ | [[Image:QQ图片20150210175655.jpeg]] | ||
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Revision as of 09:46, 16 September 2015
RBS B0030 w/ YFP (-LVA) TT
B0030. E0030. B0015
Experience
Glasgow iGEM 2011
This part was found to express well when excited with fluorescence in the range of 515-565nm. See images below for expression testing:
Shenzhen_SFLS (IGEM 2015)
We expressed this part in E.coli using pbad promoter and J23116 promoter.
From left to right: J23116/pbad(0μM L-Arabinose)/1.0μM/2.0μM/4.0μM(best in the 5 concentrations we usd)/8.0μM
File:QQ图片20150210175650.jpeg File:QQ图片20150210175655.jpeg
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]