Difference between revisions of "Part:BBa K1642010"

Revision as of 09:07, 16 September 2015

Expression of Halorhodopsin under the control of PcpcG2

PcpcG2-HR is a plasmid used to test whether the protein halorhodopsin works as we expect.

Up and Down are sequences uesd for homologous recombination.

PcpcG2 is the promoter of cpcG2. CcaS phosphorylates CcaR under green light, and phosphorylated CcaR binds to promoter of cpcG2 to induce expression of cpcG2.

RBS is the RNA binding site, which will hopefully increase the expression level of HR.

HR

Terminator

PcpcB is a constitutive promoter in cyanobacteria, and Kana is gene sequence for kanamycin resistance, so that bacteria which are successfully transferred this plasmid can be screened on the plate.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 3398
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 481
Illegal NgoMIV site found at 966
Illegal NgoMIV site found at 1125
Illegal NgoMIV site found at 1446
Illegal AgeI site found at 1317
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1642010 short</partinfo>
-Pdark-HR
+PcpcG2-HR is a plasmid used to test whether the protein halorhodopsin works as we expect.
+Up and Down are sequences uesd for homologous recombination.
+PcpcG2 is the promoter of cpcG2. CcaS phosphorylates CcaR under green light, and phosphorylated CcaR binds to promoter of cpcG2 to induce expression of cpcG2.
+RBS is the RNA binding site, which will hopefully increase the expression level of HR.
+HR
+Terminator
+PcpcB is a constitutive promoter in cyanobacteria, and Kana is gene sequence for kanamycin resistance, so that bacteria which are successfully transferred this plasmid can be screened on the plate.
 <!-- Add more about the biology of this part here