Difference between revisions of "Part:BBa K1604025:Design"
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Arabinose inducible araC-pBAD promoter come from <i>E. coli</i> genome. | Arabinose inducible araC-pBAD promoter come from <i>E. coli</i> genome. | ||
| − | Beta-carotene gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) genome. | + | Beta-carotene gene coding sequences come from <i>Pantoea ananatis</i> (formerly <i>Erwinia uredovora</i>) genome. |
Protheorodopsin come from HF10_19P19, uncutured bacteria of SAR86 group, gammaprotoeobacteria. | Protheorodopsin come from HF10_19P19, uncutured bacteria of SAR86 group, gammaprotoeobacteria. | ||
Latest revision as of 08:51, 16 September 2015
araC-pBAD + proteorhodopsin + j23100 + blh + araC-pBAD + β-Carotene
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2019
Illegal NheI site found at 2042 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1740
Illegal BamHI site found at 1144
Illegal BamHI site found at 4051
Illegal BamHI site found at 6092
Illegal XhoI site found at 1313 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2257
Illegal NgoMIV site found at 5628
Illegal NgoMIV site found at 5758
Illegal AgeI site found at 979
Illegal AgeI site found at 1502
Illegal AgeI site found at 1877
Illegal AgeI site found at 3886
Illegal AgeI site found at 4843 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Illegal SapI site found at 3868
Design Notes
Standard assembly between BBa_K1604022 and a PCR amplification of BBa_K1604010.
NOTE This device was built using a part from the Cambridge 2009 team. Be careful when operating this part, because the SpEI site in the suffix is missing. The part from Cambridge 2009 had this mistake and so the error was introduced in our part. We realized that after sequencing. Other than that the device work well.
Source
Arabinose inducible araC-pBAD promoter come from E. coli genome. Beta-carotene gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) genome. Protheorodopsin come from HF10_19P19, uncutured bacteria of SAR86 group, gammaprotoeobacteria.
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Walter, Jessica M., Derek Greenfield, Carlos Bustamante, and Jan Liphardt. "Light-powering Escherichia Coli with Proteorhodopsin." Proceedings of the National Academy of Sciences 104 (2007): 2408-2412.
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Martinez, A., A. S. Bradley, J. R. Waldbauer, R. E. Summons, and E. F. Delong. "Proteorhodopsin Photosystem Gene Expression Enables Photophosphorylation in a Heterologous Host" Proceedings of the National Academy of Sciences 104.13 (2007): 5590-595. Web.