Difference between revisions of "Part:BBa K1642009"
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Pdark-GFP is a plasmid used to test whether the promoter Pdark works as we expect. | Pdark-GFP is a plasmid used to test whether the promoter Pdark works as we expect. | ||
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+ | Up and Down are sequences uesd for homologous recombination. | ||
+ | Pdark(BBa_K1026009) is a “dark-sensing” promoter combines PcpcG2 and a constitutive promoter from ''E.coli''.Pdark contains binding site of CcaR in PcpcG2, which overlaps with the binding site of RNA polymerase. Therefore when phosphorylated CcaR binds to it, the binding of RNA polymerase to Pdark will be blocked and transcription can’t be initiated. | ||
Revision as of 08:02, 16 September 2015
Expression of GFP under the control of Pdark
Pdark-GFP is a plasmid used to test whether the promoter Pdark works as we expect.
Up and Down are sequences uesd for homologous recombination. Pdark(BBa_K1026009) is a “dark-sensing” promoter combines PcpcG2 and a constitutive promoter from E.coli.Pdark contains binding site of CcaR in PcpcG2, which overlaps with the binding site of RNA polymerase. Therefore when phosphorylated CcaR binds to it, the binding of RNA polymerase to Pdark will be blocked and transcription can’t be initiated.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 611
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2995
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 481
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1294