Difference between revisions of "Part:BBa K1859001"
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<p>BBa_K1859001 is Circular Parts conjuncted linker to keep folding of proteins.Circular Parts is what use sequence subcloned ribozyme involved self‐splicing in T4 Phage.By associating Circular Parts, the 3'side of intron[BBa_K1332005] and the 5'side of intron[BBa_K1332003], on both ends of optional sequence, you can circularize mRNA making its DNA. </p>
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<p>BBa_K1859001 is the 3'side of intron
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332005" > [BBa_K1332005] </a>
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conjuncted linker to keep folding of proteins.
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Circular Parts, the 3'side of intron  
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332005" > [BBa_K1332005] </a>
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and the 5'side of intron
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332003" > [BBa_K1332003] </a>
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, is what use sequence subcloned ribozyme involved self‐splicing in T4 Phage.
 +
 
 +
By associating Circular Parts on both ends of optional sequence, you can circularize mRNA making its DNA. </p>
 +
 
 
<p>As for details, refer to  
 
<p>As for details, refer to  
 
<a href= "http://2014.igem.org/Team:Gifu" > iGEM Gifu 2014 </a>
 
<a href= "http://2014.igem.org/Team:Gifu" > iGEM Gifu 2014 </a>
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<p>In the study of iGEM Gifu 2014, we made long-chain protein from cyclic mRNA, but it lost functionality.It is thought that folding of the protein failed for a cause．Therefore we designed some linkers to fix the folding of the protein.These parts include sequence of Circular Parts and linker．The linker comes to be in the cyclic mRNA when mRNA is circularized.To make this device, we coupled the sequence of the linker, GGSGGS , to the down stream of 3'intron.</p>
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 +
<p>In the study of iGEM Gifu 2014, we made long-chain protein from cyclic mRNA, but it lost functionality.
 +
 
 +
It is thought that folding of the protein failed for a cause．
 +
 
 +
Therefore we designed some linkers to fix the folding of the protein.
 +
 
 +
BBa_K1859001 includes sequence of the 3'side of intron
 +
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332005" > [BBa_K1332005] </a>
 +
and linker．
 +
 
 +
The linker comes to be in the Circular mRNA when mRNA is circularized.
 +
 
 +
To make this device, we coupled the sequence of the linker, GGSGGS , to the down stream of 3'side of the intron
 +
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332005" > [BBa_K1332005] </a>
 +
.</p>
 +
 
 
<p>The linker designed elsewhere is  
 
<p>The linker designed elsewhere is  
 
<a href= "http://2015.igem.org/Team:Gifu/Result/" > here </a>.</p>
 
<a href= "http://2015.igem.org/Team:Gifu/Result/" > here </a>.</p>

Revision as of 07:54, 16 September 2015

The 3´side of the intron [BBa_K1332005] +GGSGGS linker


BBa_K1859001 is the 3'side of intron [BBa_K1332005] conjuncted linker to keep folding of proteins. Circular Parts, the 3'side of intron [BBa_K1332005] and the 5'side of intron [BBa_K1332003] , is what use sequence subcloned ribozyme involved self‐splicing in T4 Phage. By associating Circular Parts on both ends of optional sequence, you can circularize mRNA making its DNA.

As for details, refer to iGEM Gifu 2014


In the study of iGEM Gifu 2014, we made long-chain protein from cyclic mRNA, but it lost functionality. It is thought that folding of the protein failed for a cause． Therefore we designed some linkers to fix the folding of the protein. BBa_K1859001 includes sequence of the 3'side of intron [BBa_K1332005] and linker． The linker comes to be in the Circular mRNA when mRNA is circularized. To make this device, we coupled the sequence of the linker, GGSGGS , to the down stream of 3'side of the intron [BBa_K1332005] .

The linker designed elsewhere is here .