Difference between revisions of "Part:BBa K1682017"
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==Usage and Biology== | ==Usage and Biology== | ||
− | In order to investigate the effect of parallel sensing, the dose response P<sub>araBAD</sub>-GFP in pSB3K3-<partinfo>BBa_K1682017</partinfo> was compared with that in pSB3K3-<partinfo>BBa_I13540</partinfo> in <i>E. coli</i> DH10B. | + | In order to investigate the effect of parallel sensing, the dose response P<sub>araBAD</sub>-GFP in pSB3K3-<partinfo>BBa_K1682017</partinfo> (double construct) was compared with that in pSB3K3-<partinfo>BBa_I13540</partinfo> (single construct) in <i>E. coli</i> DH10B. |
+ | |||
+ | [[File:Parallel_expression_BBa_K1682017_fig2.jpg|800px|centre|]] | ||
+ | '''Figure 2. Response curve of P<sub>araBAD</sub>-GFP in a single construct and double construct under varying L-Arabinose concentration.'''The different blue curves indicate different induction level of the co-expressed PlacZ-mRFP construct. At varying the induction level of the co-expressed P<sub>lac</sub>-mRFP construct seems to have an effect on the expression level of P<sub>araBAD</sub>-GFP in a double construct. At a higher IPTG concentration, the maximum expression of P<sub>araBAD</sub>-GFP decreases. | ||
Revision as of 03:41, 16 September 2015
ParaBAD-GFP-Plac-RFP
This composite part is consists of a L-Arabinose inducible ParaBAD with GFP reporter BBa_I13540, and an IPTG inducible Plac with RFP reporter Part:BBa_K1682016. The part was designed to investigate the posible effect of parallel sensing within a single plasmid.
Mechanism
Figure 1. Schematic diagram of the inducible ParaBAD-GFP-Plac-mRFP construct. The system can sense L-Arabinose to give GFP output or sense IPTG to give RFP output.
Usage and Biology
In order to investigate the effect of parallel sensing, the dose response ParaBAD-GFP in pSB3K3-BBa_K1682017 (double construct) was compared with that in pSB3K3-BBa_I13540 (single construct) in E. coli DH10B.
Figure 2. Response curve of ParaBAD-GFP in a single construct and double construct under varying L-Arabinose concentration.The different blue curves indicate different induction level of the co-expressed PlacZ-mRFP construct. At varying the induction level of the co-expressed Plac-mRFP construct seems to have an effect on the expression level of ParaBAD-GFP in a double construct. At a higher IPTG concentration, the maximum expression of ParaBAD-GFP decreases.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NheI site found at 3185
Illegal NheI site found at 3208 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4386
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 2882
Illegal AgeI site found at 2994 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1880
Illegal SapI site found at 961