Difference between revisions of "Part:BBa K1682017"
Line 2: Line 2:
 
<partinfo>BBa_K1682017 short</partinfo>
 
<partinfo>BBa_K1682017 short</partinfo>
  
−
This composite part is consists of a L-Arabinose inducible Pbad with GFP reporter, and an IPTG inducible Plac with RFP reporter.  
+
This composite part is consists of a L-Arabinose inducible P<sub>araBAD</sub> with GFP reporter <partinfo>BBa_I13540</partinfo>, and an IPTG inducible P<sub>lac</sub> with RFP reporter [https://parts.igem.org/Part:BBa_K1682016 Part:BBa_K1682016].
 
The part was designed to investigate the posible effect of parallel sensing within a single plasmid.  
 
The part was designed to investigate the posible effect of parallel sensing within a single plasmid.  
  
−
<!-- Add more about the biology of this part here
+
==Mechanism==
−
===Usage and Biology===
+
[[File:Parallel_expression_BBa_K1682017.jpg|700px|centre|]]
 +
'''Figure 1. Schematic diagram of the inducible P<sub>araBAD</sub>-GFP-P<sub>lac</sub>-mRFP construct.''' The system can sense L-Arabinose to give GFP output or sense IPTG to give RFP output.
 +
 
 +
==Usage and Biology==
 +
In order to investigate the effect of parallel sensing, the dose response P<sub>araBAD</sub>-GFP in pSB3K3-<partinfo>BBa_K1682017</partinfo> was compared with that in pSB3K3-<partinfo>BBa_I13540</partinfo> in <i>E. coli</i> DH10B.
 +
 
  
−
<!-- -->
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1682017 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1682017 SequenceAndFeatures</partinfo>

Revision as of 03:13, 16 September 2015

ParaBAD-GFP-Plac-RFP

This composite part is consists of a L-Arabinose inducible ParaBAD with GFP reporter BBa_I13540, and an IPTG inducible Plac with RFP reporter Part:BBa_K1682016. The part was designed to investigate the posible effect of parallel sensing within a single plasmid.

Mechanism

Parallel expression BBa K1682017.jpg

Figure 1. Schematic diagram of the inducible ParaBAD-GFP-Plac-mRFP construct. The system can sense L-Arabinose to give GFP output or sense IPTG to give RFP output.

Usage and Biology

In order to investigate the effect of parallel sensing, the dose response ParaBAD-GFP in pSB3K3-BBa_K1682017 was compared with that in pSB3K3-BBa_I13540 in E. coli DH10B.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 3185
    Illegal NheI site found at 3208
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4386
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2882
    Illegal AgeI site found at 2994
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1880
    Illegal SapI site found at 961