Difference between revisions of "Part:BBa K1675026:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K1675026 short</partinfo>
 
<partinfo>BBa_K1675026 short</partinfo>
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===Source===
 
===Source===
  
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Error prone PCR of P-atp2, then overlap extension of synthetic oligonucleotides.
 
  
 
The natural P-atp2 is from Corynebacterium glutamicum.
 
The natural P-atp2 is from Corynebacterium glutamicum.

Latest revision as of 18:31, 15 September 2015

P-atp2-B0034-lacz alpha


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is an EcoRI site exists in the P-atp2 sequence, so we mutate it to change it.


Source

The natural P-atp2 is from Corynebacterium glutamicum.

Lacz alpha is from https://parts.igem.org/wiki/index.php?title=Part:BBa_I732006


References