Difference between revisions of "Part:BBa K1723006:Design"
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
Revision as of 17:28, 15 September 2015
sgRNA X0 expressing cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 126
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was design on the model for sgRNAs on the paper from Alec AK Nielsen & Christopher A Voigt [3] and the specific sequence for the gRNA Z0 was designed from the sequence of the PAM rich URS J23117Alt promoter.
Source
This sequence was fully synthesized. [edit]