Difference between revisions of "Part:BBa K1723004"

Revision as of 16:35, 15 September 2015

sgRNA Z35 expressing cassette

This part is expressing the sgRNA (single guide RNA) Z35 that can bind to dCas9-ω (BBa_K1723000). The complex was designed to bind the PAM rich URS J23117 promoter (BBa_K1723001) to activate the production of the target gene linked to the promoter. This part is a part of a gene regulation system built with dCas9-ω, with the parts sgRNA Z4 expressing cassette (BBa_K1723003) and gRNA Z0 expressing cassette (BBa_K1723002). This sgRNA coding sequence was biobricked with the promoter pBAD and its terminator, both used for our experiments. As the gRNA sequence has to start the first base after the promoter it couldn't have been used with the standard biobrick system so the promoter is provided already. It is possible to PCR out the part to put it under another promoter, but he terminator is specific and it is recommended to keep it.

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http://2015.igem.org/Team:EPF_Lausanne/Part_Collection

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 178
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 125
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1723004 short</partinfo>
-This part is expressing the sgRNA (single guide RNA) Z0 that can bind to dCas9-&#969; (BBa_K1723000). The complex was designed to bind the PAM rich URS J23117 promoter (BBa_K1723001) to activate the production of the target gene linked to the promoter. This part is a part of a gene regulation system built with dCas9-&#969;, with the parts sgRNA Z4 expressing cassette (BBa_K1723003) and gRNA Z35 expressing cassette (BBa_K1723004). This sgRNA coding sequence was biobricked with the promoter pBAD and its terminator, both used for our experiments. As the gRNA sequence has to start the first base after the promoter it couldn't have been used with the standard biobrick system so the promoter is provided already. It is possible to PCR out the part to put it under another promoter, but he terminator is specific and it is recommended to keep it.
+This part is expressing the sgRNA (single guide RNA) Z35 that can bind to dCas9-&#969; (BBa_K1723000). The complex was designed to bind the PAM rich URS J23117 promoter (BBa_K1723001) to activate the production of the target gene linked to the promoter. This part is a part of a gene regulation system built with dCas9-&#969;, with the parts sgRNA Z4 expressing cassette (BBa_K1723003) and gRNA Z0 expressing cassette (BBa_K1723002). This sgRNA coding sequence was biobricked with the promoter pBAD and its terminator, both used for our experiments. As the gRNA sequence has to start the first base after the promoter it couldn't have been used with the standard biobrick system so the promoter is provided already. It is possible to PCR out the part to put it under another promoter, but he terminator is specific and it is recommended to keep it.
 Discover all the parts that can work with this one: