Difference between revisions of "Part:BBa K1723001"

Revision as of 15:55, 15 September 2015

PAM rich URS j23117 promoter

PAM rich URS J23117 is a promoter made from the existing J23117 (BBa_J23117) weak promoter. This promoter has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-ω (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA targeting the promoter such as: Z0 (BBa_K1723002), Z35 (BBa_K1723003), or Z4 (BBa_K1723004).

Discover all the parts that can work with this one:

http://2015.igem.org/Team:EPF_Lausanne/Part_Collection

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 56
Illegal NheI site found at 79
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 43
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 PAM rich URS J23117 is a promoter made from the existing J23117 (BBa_J23117) weak promoter. This promoter has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-&#969; (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA targeting the promoter such as: Z0 (BBa_K1723002), Z35 (BBa_K1723003), or Z4 (BBa_K1723004).
+Discover all the parts that can work with this one:
+http://2015.igem.org/Team:EPF_Lausanne/Part_Collection
 https://static.igem.org/mediawiki/2015/f/f9/EPFL_Lausanne_promoter.png