Difference between revisions of "Part:BBa K1587006"
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Uses a positively regulated promoter (pompC) and a lacI binding site to regulate the expression of cI. This construction was designed to be regulated by cph8 red light receptor (cph1-envZ chimeric protein). The purpose was to create a circadian switch to alternate the expression of two sets of genes. | Uses a positively regulated promoter (pompC) and a lacI binding site to regulate the expression of cI. This construction was designed to be regulated by cph8 red light receptor (cph1-envZ chimeric protein). The purpose was to create a circadian switch to alternate the expression of two sets of genes. | ||
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[[File:TLSE-Circadian.JPG]] | [[File:TLSE-Circadian.JPG]] | ||
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Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol. | Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol. | ||
Revision as of 13:59, 15 September 2015
pompC and lacI box regulated cI expression
Uses a positively regulated promoter (pompC) and a lacI binding site to regulate the expression of cI. This construction was designed to be regulated by cph8 red light receptor (cph1-envZ chimeric protein). The purpose was to create a circadian switch to alternate the expression of two sets of genes.
Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]