Difference between revisions of "Part:BBa K1632013:Experience"

(→‎Materials and Methods)
(→‎Materials and Methods)
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All the samples were DH5α strain with antibiotic resistance to ampicillin and kanamycin.<br>
 
All the samples were DH5α strain with antibiotic resistance to ampicillin and kanamycin.<br>
  
−
A. PBAD/araC_FimE(wild-type) (6A1) +fim switch[default ON](wild-type)_gfp (3K3) <br>
+
A. PBAD/araC_fimE(wild-type) (6A1) +''fim'' switch[default ON](wild-type)_rbs_gfp (3K3) <br>
−
B. PBAD/araC_FimE(wild-type) (6A1) +fim switch[default ON](wild-type)_gfp (3K3) <br>
+
B. PBAD/araC_fimE(wild-type) (6A1) +''fim'' switch[default OFF](wild-type)_rbs_gfp (3K3) <br>
−
C. rbs_M256IcysE (6A1) + fim switch[default ON](wild-type)_gfp (3K3) …positive control 1<br>
+
C. rbs_M256IcysE (6A1) + ''fim'' switch[default ON](wild-type)_rbs_gfp (3K3) …positive control 1<br>
−
D. rbs_M256IcysE (6A1) + fim switch[default OFF](wild-type)_gfp (3K3) …negative control 1<br>
+
D. rbs_M256IcysE (6A1) + ''fim'' switch[default OFF](wild-type)_rbs_gfp (3K3) …negative control 1<br>
−
E. PBAD/araC_FimE(wild-type) (6A1) + J23119_gfp (3K3) …positive control 2 <br>
+
E. PBAD/araC_fimE(wild-type) (6A1) + J23119_rbs_gfp (3K3) …positive control 2 <br>
−
F. PBAD/araC_FimE(wild-type) (6A1)+rbs_gfp (3K3) …negative control 2 <br>
+
F. PBAD/araC_fimE(wild-type) (6A1)+rbs_gfp (3K3) …negative control 2 <br>
 
[[Image:Tokyo_Tech_FimE_assay.png|thumb|center|600px|<b>Fig. 1. </b>Plasmids]]<br>
 
[[Image:Tokyo_Tech_FimE_assay.png|thumb|center|600px|<b>Fig. 1. </b>Plasmids]]<br>
  
 
<b>2. Assay protocol</b><br>
 
<b>2. Assay protocol</b><br>

Revision as of 13:48, 15 September 2015

PBAD/araC_rbs_fimE(wild-type)

Materials and Methods

1. Construction
All the samples were DH5α strain with antibiotic resistance to ampicillin and kanamycin.

A. PBAD/araC_fimE(wild-type) (6A1) +fim switch[default ON](wild-type)_rbs_gfp (3K3)
B. PBAD/araC_fimE(wild-type) (6A1) +fim switch[default OFF](wild-type)_rbs_gfp (3K3)
C. rbs_M256IcysE (6A1) + fim switch[default ON](wild-type)_rbs_gfp (3K3) …positive control 1
D. rbs_M256IcysE (6A1) + fim switch[default OFF](wild-type)_rbs_gfp (3K3) …negative control 1
E. PBAD/araC_fimE(wild-type) (6A1) + J23119_rbs_gfp (3K3) …positive control 2
F. PBAD/araC_fimE(wild-type) (6A1)+rbs_gfp (3K3) …negative control 2

Fig. 1. Plasmids

2. Assay protocol