Difference between revisions of "Part:BBa K1864000"
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We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours | We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours | ||
[[File:FAFU-CHINA PART (1).png|200px|thumb|left|Figure 1.Concentration of dsRdRp differs under different concentration of IPTG. The concentration of dsRdRp continues to increase till the concentration of IPTG reaches to 0.4 mmol/L.]] | [[File:FAFU-CHINA PART (1).png|200px|thumb|left|Figure 1.Concentration of dsRdRp differs under different concentration of IPTG. The concentration of dsRdRp continues to increase till the concentration of IPTG reaches to 0.4 mmol/L.]] | ||
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<h3>The inductive effect of different time length</h3> | <h3>The inductive effect of different time length</h3> | ||
We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration. | We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration. | ||
[[File:FAFU-CHINA PART (2).png|200px|thumb|left|Figure 2.Concentration of dsRdRp changes with three different concentration of IPTG over time. Induced by IPTG about 4 hours, the concentration of dsRdRp appears to decrease or remain unchanged]] | [[File:FAFU-CHINA PART (2).png|200px|thumb|left|Figure 2.Concentration of dsRdRp changes with three different concentration of IPTG over time. Induced by IPTG about 4 hours, the concentration of dsRdRp appears to decrease or remain unchanged]] | ||
Revision as of 13:30, 15 September 2015
CSBV-RdRP
Our part is CSBV's RdRP,RdRP gene cloned into the L4440 vector,and transformed into E.coli strain HT115 to express dsRdRP.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 837
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 110
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Application
Group: FAFU-CHINA
Author: Ruicheng Dai & Changlong Lu
Summary: The control efficiency of dsRdRp in Chinese Scabrood Virus（CSBV）
Design
In our project,we have been aiming to control CSBV through silence of CSBV’s RdRp (RNA-dependent RNA polymerase) gene by using RNA-interference technology. We built recombinant L4440 plasmids which contained dual T7 promoter and we transformed it to HT115 strain. After the enlage cultivation of HT115, we extracted dsRdRp from bacteria. And we added different concentrations into the honeybees' food. Meanwhile,We noticed that the induction of IPTG could improve the expression of dsRdRp. Therefore,we also tested the inductive effect of IPTG.
The control efficiency of dsRdRp in Chinese Scabrood Virus
In apiculture, scientists regard the number of sealed brood as an important index which means normal developing embryo.Therefore,we gathered datas about the effects in different dsRdRp concentrations and administration time.
File:FAFU-CHIAN Parts information (1).png
IPTG inductive effect
We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect.
The inductive effect of different concentration IPTG
We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours
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The inductive effect of different time length
We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration.
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