Difference between revisions of "Part:BBa K1723001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | PAM rich sequence was a bit shortened comparing to the original promoter in pWJ89 plasmid as it contained one RFC10 restriction site but all the binding sites remains intact. | + | PAM rich sequence was a bit shortened comparing to the original promoter in pWJ89 plasmid as it contained one RFC10 restriction site but all the binding sites remains intact. In our experiments this promoter is 27 base pairs upstream the ATG start codon. |
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===Source=== | ===Source=== | ||
Revision as of 12:36, 15 September 2015
PAM rich URS j23117 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 56
Illegal NheI site found at 79 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 43
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PAM rich sequence was a bit shortened comparing to the original promoter in pWJ89 plasmid as it contained one RFC10 restriction site but all the binding sites remains intact. In our experiments this promoter is 27 base pairs upstream the ATG start codon.
Source
This promoter was shipped to us by Dr David Bikard, from the pasteur institute, in the pWJ89 plasmid. [1]
References
[1] Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013). Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic acids research, 41(15), 7429-7437.