Difference between revisions of "Part:BBa K1724001"
Line 2: Line 2:
 
<partinfo>BBa_K1724001 short</partinfo>
 
<partinfo>BBa_K1724001 short</partinfo>
  
−
The mercury –sensing regulatory protein, MerR(wild type), which regulates mercury resistance operons in Gram-negative bacteria, is subjected to directed evolution in an effect to generate a MerR mutant that responds to Cadmium ion but not mercury.[1]That is, the MerR mutant is the cadmium-sensing regulatory protein. To get MerR mutant, Oligonucleotide-directed mutagenesis is used to introduce random mutations into the key metal-binding regions of MerR. Finally, Getting the generated Cd-specific MerR mutants appears to be unique.
+
The mercury –sensing regulatory protein, MerR(wild type), which regulates mercury resistance operons in Gram-negative bacteria, is subjected to directed evolution in an effect to generate a MerR mutant that responds to Cadmium ion but not mercury.That is, the MerR mutant is the cadmium-sensing regulatory protein. To get MerR mutant, Oligonucleotide-directed mutagenesis is used to introduce random mutations into the key metal-binding regions of MerR. Finally, Getting the generated Cd-specific MerR mutants appears to be unique.
  
 
The quantification data of MerR/CadA operon can be seen in  <html><a href="https://parts.igem.org/Part:BBa_K1724000"target="_blank">BBa_K1724000</a>
 
The quantification data of MerR/CadA operon can be seen in  <html><a href="https://parts.igem.org/Part:BBa_K1724000"target="_blank">BBa_K1724000</a>
  
−
== Reference ==
 
−
 
−
1.Hakkila KM, Nikander PA, Junttila SM, Lamminmaki UJ, Virta MP: Cd-Specific Mutants of Mercury-Sensing Regulatory Protein MerR, Generated by Directed Evolution. Applied and environmental microbiology 2011, 77(17):6215-6224.
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:56, 15 September 2015

RBS+CsgA

The mercury –sensing regulatory protein, MerR(wild type), which regulates mercury resistance operons in Gram-negative bacteria, is subjected to directed evolution in an effect to generate a MerR mutant that responds to Cadmium ion but not mercury.That is, the MerR mutant is the cadmium-sensing regulatory protein. To get MerR mutant, Oligonucleotide-directed mutagenesis is used to introduce random mutations into the key metal-binding regions of MerR. Finally, Getting the generated Cd-specific MerR mutants appears to be unique.

The quantification data of MerR/CadA operon can be seen in BBa_K1724000 Sequence and Features BBa_K1724001 SequenceAndFeatures